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Analyzing mechanical traits of engineered heart tissueMeasurement of tissue movement with POC-based analysis 
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Recently, research aiming to elucidate the causes and therapies of diseases using artificial tissues derived from disease-specific induced pluripotent stem cells has been active. An important part of this approach is to quantitatively measure the functional health or performance of the artificial tissue of interest. Specifically, in the engineered heart tissue (EHT), it is very important to measure its contraction and relaxation forces. To this end, we develop a method based on the Phase-only Correlation for measuring contractile and diastolic forces by culturing cardiomyocytes on a pair of pillars with known mechanical properties and capturing video of tissue movement in response to a cyclic stimulus using a fluorescence microscope. The method tracks the tissue movement over the frames and then determines its maximum deflection and frequency by fitting the detected deflection to a sine function. We show that our method enables the measurement of the mechanical performance of the EHT.
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[3] M. Tsuchida, M. Hasegawa, K. Miki, S. Miyagawa, K. Kashino, “Mechanical performance of engineered heart tissue can be measured with POC-based video analysis,” in Proc. The 46th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC), under review.
Masaru Tsuchida, Biomedical Informatics Research Group, Media Information Laboratory